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청정도검사설비
BX41
 
Virtual Microscope VS120
 
Key Feature

VS120 Fluorescence

Fluorescent markers have become one of the main tools in the generation of clear contrast in light microscopy, enabling the simple visualization of molecules, organelles and cell types for ultra-precise localisation and transport studies. The VS120 virtual slide system can be used with fluorescent samples, giving a completely unique perspective - a slide recorded in full fluorescence can be thoroughly examined without any risk of bleaching or damage to the cells. All VS120 fluorescence system components are designed to interact seamlessly, producing a fully automated, high-speed multi-channel fluorescence scanning system with excellent flexibility and simple operation.
 
Specifications
Hardware Camera 2/3” CCD camera, 6.45 μm x 6.45 μm pixel size, high sensitivity, Peltier cooled, dynamic range of 14 bit
Objectives Any Olympus UIS2 objective suitable for fluorescence (including the standard objectives already present in the VS120 systems like PLAPON 2x, UPLSAPO 10, UPLSAPO 20x, UPLSAPO 40x)
Light Source X-Cite® exacte from Lumen Dynamics, Closed-loop Feedback stabilized intensity, light output fully controllable in 1% steps, long lamp lifetime (2000 hours), light guide coupling to microscope frame, pre-aligned lamp
Filters Ultra-fast excitation filter wheel (8 position, standard 25mm filter holders), 6 position motorized dicroic filter holder. Different multi-band filter sets available based on user needs. Optional Ultra-fast emission filter wheel (8 positions, standard 25mm filter holders) for high selectivity and increased S/N Ratio
Resolution

0.32 μm/pixel (20x/NA 0.75) and 0.16 μm/pixel (40x/NA 0.9)
Scanning Speed
(10x10 mm area, 20X magnification)

< 5 min with 2 channels

Overall fluorescence scanning times vary depending on the intensity of the sample staining, number of color channels and scanning area
Options

Any Olympus UIS2 objective suitable for fluorescence (in addition to the standard objectives already present in the VS120 systems)

Software Fluorescence Image Acquisition
  • Additional wizards dedicated to fluorescence acquisitions
  • Real-time single-plane deconvolution of acquired images (Online Deblur)
  • Combined fluorescence/brightfield scanning, including DIC and Phase contrast methods (with proper optical accessories)
  • Seamless stitching
  • Virtual Z-Acquisition
  • Fluorescence Batch Scan
  • User defined acquisition parameter sets (Scan Projects), easy multi user acquisitions and true standardized scans for quantitative analysis
  • Fluorescence autofocus channel selection (e.g. autofocus on DAPI, scan on FITC+TRITC)
  • Independent z-offsets for each fluorescence channel, based on found focus at each position
  • Independent Shading Correction for each fluorescence channel
Software Functions
  • View images locally, zoom, crop and rotate  images
  • Select/exclude individual fluorochrome/brightfield channels for viewing or side-by-side comparison
  • Extract subset of original scan (specific X and Y area, Z-interval, individual channels)
  •   Compress images and save images in different file formats
  • Insert text annotations and drawings; create  views of regions of interest, record audio annotations(sound card, microphone and speakers required)